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簡(jiǎn)要描述：Vala Sciences Screen-Certified (Z'> 0.5) Cell-Imaging Kits include fixative, permeabilization, and staining solutions in amounts sufficient to stain cells cultured in five 96-well dishes.

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Vala Sciences Screen-Certified （Z'> 0.5） Cell-Imaging Kits include fixative, permeabilization, and staining solutions in amounts sufficient to stain cells cultured in five 96-well dishes.

Anti-perilipin Mouse Monoclonal Antibody

4998

#4854

Given the ever increasing interest in elucidating the mechanisms that regulate lipolysis, Vala Sciences Inc is proud to introduce a new monoclonal antibody that is excellent at visualizing perilipin in immunocytofluorescence labeling applications. This new mouse monoclonal antibody recognizes perilipin whether or not lipolytic pathways have been activated in the cell. Labeling with the antibody is exceptionally specific, with virtually no background, and the labeling pattern is very tightly associated with the outer surface of the lipid droplets, as expected for perilipin. This antibody is expected to be of high interest to researchers investigating hormonal control of lipolysis.

100 ul

Anti-perilipin mouse IgG1 monoclonal
immunogen: peptide near serine 497 of human perilipin used for Ab generation but Ab not specific to S497 phosphorylation.
species recognized: human, mouse. full-length Perilipin A

Available volume discounts and contract testing services

Anti-phosphoperilipin Mouse Monoclonal Antibody （Serine 497）

6188

#4855

Given the ever increasing interest in elucidating the mechanisms that regulate lipolysis, Vala Sciences Inc is proud to introduce a new monoclonal antibody that is excellent at visualizing perilipin in immunocytofluorescence labeling applications. This monoclonal antibody selectively recognizes perilipin that has been phosphorylated on serine 497, a site of PKA-mediated phosphorylation. Thus, for the first time, a tool is available in which phosphorylated perilipin can be visualized in fixed cells via immunocytofluorescence labeling procedures and fluorescence microscopy. This antibody is expected to be of high interest to researchers investigating hormonal control of lipolysis.

100 ul

Anti-Phosphoperilipin mouse IgG1 monoclonal
immunogen: peptide near serine 497 of human perilipin
species recognized: human, mouse. full-length Perilipin A

Available volume discounts and contract testing services

Anti-phosphoperilipin Mouse Monoclonal Antibody （Serine 522）

6188
#4856

Given the ever increasing interest in elucidating the mechanisms that regulate lipolysis, Vala Sciences Inc is proud to introduce a new monoclonal antibody that is excellent at visualizing perilipin in immunocytofluorescence labeling applications. This monoclonal antibody selectively recognizes perilipin that has been phosphorylated on serine 522, a site of PKA-mediated phosphorylation. Thus, for the first time, a tool is available in which phosphorylated perilipin can be visualized in fixed cells via immunocytofluorescence labeling procedures and fluorescence microscopy. This antibody is expected to be of high interest to researchers investigating hormonal control of lipolysis.

100 ul

Anti-Phosphoperilipin mouse IgG1 monoclonal
immunogen: peptide near serine 522 of human perilipin
species recognized: human, mouse. full-length Perilipin A

Available volume discounts and contract testing services

b-catenin Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

12750元
#4807

b-catenin, the mammalian homologue of Drosophila Armadillo, is a dual purpose protein of high interest to biomedical researchers concerned with tumor formation. In normal epithelial cells, b-catenin is found at the plasma membrane. b-catenin expression and localization can be quantified in cultured cells with Vala Sciences Inc.'s b-catenin kit.

E-Cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

11900
#4803

E-cadherin, a cell-cell adhesion molecule, is frequently downregulated in a wide variety of tumors （breast, skin, prostate, gastric, and colon cancers）, suggesting that it is an important tumor suppressor. E-cadherin is the major binding partner for -catenin, and E-cadherin downregulation is often accompanied by beta-catenin translocation to the nucleus, and the stimulation of cell division. E-cadherin expression and localization can be quantified in cultured cells with Vala Sciences Inc's E-cadherin kit.

Lipid Droplet Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

3740元
#4805

Lipid droplets are the primary fat storage organelles of fat cells （adipocytes）. Lipid droplet size, and number are altered by anti-diabetic and anti-obesity drugs and these effects can be quantified utilizing Vala Science Inc's Lipid Droplet Kit. The kit can also be utilized to quantify lipid droplets in murine 3T3 L1 adipocytes.

N-Cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

7395

#4802

N-cadherin, a cell-cell adhesion molecule, is upregulated in many tumor types （including breast, ovarian, prostate, and skin cancers） and may be involved in metastastic potential. N-cadherin often replaces E-cadherin during tumorigenesis, a phenomenon referred to as the "cadherin switch". N-cadherin expression and cellular localization can be quantified in cultured cells with Vala Sciences Inc's N-cadherin kit.

Pan-Cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5, Mouse mAb-488）

6460
#4812

Accurate quantitation of cadherin expression and localiztion as they are related to cell adhesion, tissue structure and mitotic signaling are valuable across a variety of biomedical contexts including cell differentiation, embryonology, neurobiology, vascular pharmacology, and oncology. Vala Sciences' Pan-cadherin assay kits, used in conjunction with Vala's CyteSeer® automated cell image analysis software, enable precise quantification of cadherin expression and localization.

Pan-Cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5, Mouse mAb-547）

4420

#4808

Pan-Cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5, Rabbit Poly-488）

5100
#4809

Pan-Cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5, Rabbit Poly-547）

5100

#4810

Perilipin Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

16150
#4806

Perilipin is a protein that associates with lipid droplets in mature adipocytes and is involved in lipid droplet metabolism. The association of perilipin with lipid droplets in primary human adipocytes can be quantified through use of Vala Science Inc's Perilipin kit.

Download the product insert

pHSLserine660 （Vala#4820）

11815

#4820

The reagents and protocol in this kit will specifically visualize Hormone Sensitive Lipase （HSL）, which has been phosphorylated on serine 660 （referenced to the sequence of rat HSL）. Phosphorylation of HSL on serine 660 occurs as HSL is activated in adipocytes exposed to agents that stimulate lipolysis of triglycerides. Reagents in this kit have been validated with both human and murine adipocytes, and will visualize pHSL-serine 660 in the red fluorescence channel. Lipid droplets will also be labeled, and will be visible in the green fluorescence channel.

PKC-Alpha Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

5780
#4801

Protein kinase Cα（PKCα）, a key regulatory protein in signal transduction pathways modulating cancer and inflammation, is activated via G-protein coupled receptors, tumor promoting chemicals, and other stimuli. Activation of PKCa depends upon migration of PKCa from the cytoplasm to the cell membrane. The expression and activation of this important signal transduction molecule can be quantified in cultured cells （validated for HeLa, UMR106, or IEC18 cells）, with Vala Sciences Inc's PKCa kit.

pPerilipin-serine497 + pHSLser660 + Lipid droplets （Vala #4822）

16915

#4822

The reagents in this kit will specifically visualize triglyceride containing lipid droplets, Perilipin A （the most common form of Perilipin in adipocytes）, which has been phosphorylated on serine 497 （human sequence, equivalent to mouse serine 492, also known as PKA phosphorylation site #5）, and Hormone Sensitive Lipase （HSL）, which has been phosphorylated on serine 660 （referenced to the sequence of rat HSL）. Phosphorylation of perilipin on serine 497 and HSL on serine 660 occurs early in the activation scheme of lipolysis in adipocytes. The reagents in this kit therefore provide tools for visualizing the appearance of two key regulators of lipid droplet metabolism （pPerilipin-serine497 and pHSL-serine660）, as well as the lipid droplets, themselves. Reagents in this kit have been validated with both human and murine adipocytes.

VE-cadherin Screen-Certified Cell-Imaging Kit （Z’ > 0.5）

9350
#4804

VE-cadherin is expressed by vascular endothelial cells where it regulates cell-cell adhesion, angiogenesis, and vascular permeability. VE-cadherin is upregulated in breast cancer and may participate in tumor-associated angiogenesis. VE-cadherin expression and localization can be quantified in cultured cells with Vala Sciences Inc's VE-cadherin kit.

Definition of Z’: For high throughput assay development and validation, a statistic has been defined （Z’） which provides a quantitative index of the relationship between the sample to sample variability and the range of the assay （Zhang et al., 1999）. Z’ = 1-3（SDmax+SDmin）/（Xmax-Xmin） where SDmax and SDmin are the standard deviations of the maximum and minimum value samples, respectively, and Xmax and Xmin are the means of the maximum and minimum, respectively.

Z’ has a theoretic range of negative infinity to 1.0 （an ideal assay in which there is no standard deviation and finite separation between means would yield Z’ = 1.0）. A positive Z’ score is much more stringent than a p < 0.05 determination from a t-test. Z’ scores > 0.5 are excellent and indicate that an there is an extremely low probability of obtaining ‘false positives’ such assays can be used, with very high confidence, to screen libraries representing hundreds of thousands of test compounds.

Zhang, J.H., Chung, T.D. & Oldenburg, K.R. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J Biomol Screen 4, 67-73 （1999）.

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